Spanish Research Report for 1994

13 páginas, 12 tablasPeer reviewe

Greenland Halibut Depth Variations of Catch-per-unit Effort, Length Composition, Mature Proportions and Associated By-catches in Divisions 3LMNO

In this paper we present a review of the geographic and bathimetric patterns of occurrence of the Greenland halibut catches in NAFO Divisions 3LMNO and their incidence on the catch of other stocks, namely cod, American plaice and yellowtail and witch flounders. The Greenland halibut mature proportions at depth in both the commercial catches and the spring Spanish 3NO survey indices are also presented. According to the results of the Spanish fleet, the Greenland halibut fishery does not overlap significantly with either American plaice, yellowtail flounder, skate or cod. By-catches of witch flounder in the Greenland halibut fishery have been very small, at least in 1999. The CPUE analysis indicate that Greenland halibut is best caught in the deepest strata, with catch rates comparatively poor at depths less than 600 m. Besides a sharp change in catchability is observed between 500 – 600 m. The proportion of mature fish in the catches increases with depth attaining a maximum between 1200 – 1600 m. The survey results indicate that most part of the SSB is found at depths beyond 800 m

Spanish Research Report for 1998

11 páginas, 9 tablasPeer reviewe

Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways

Mammalian sperm acquire fertilizing ability in the female tract in a process known as capacitation. At the molecular level, capacitation is associated with up-regulation of a cAMP-dependent pathway, changes in intracellular pH, intracellular Ca2+, and an increase in tyrosine phosphorylation. How these signaling systems interact during capacitation is not well understood. Results presented in this study indicate that Ca2+ ions have a biphasic role in the regulation of cAMP-dependent signaling. Media without added Ca2+ salts (nominal zero Ca2+) still contain micromolar concentrations of this ion. Sperm incubated in this medium did not undergo PKA activation or the increase in tyrosine phosphorylation suggesting that these phosphorylation pathways require Ca2+. However, chelation of the extracellular Ca2+ traces by EGTA induced both cAMP-dependent phosphorylation and the increase in tyrosine phosphorylation. The EGTA effect in nominal zero Ca2+ media was mimicked by two calmodulin antagonists, W7 and calmidazolium, and by the calcineurin inhibitor cyclosporine A. These results suggest that Ca2+ ions regulate sperm cAMP and tyrosine phosphorylation pathways in a biphasic manner and that some of its effects are mediated by calmodulin. Interestingly, contrary to wild-type mouse sperm, sperm from CatSper1 KO mice underwent PKA activation and an increase in tyrosine phosphorylation upon incubation in nominal zero Ca2+ media. Therefore, sperm lacking Catsper Ca2+ channels behave as wild-type sperm incubated in the presence of EGTA. This latter result suggests that Catsper transports the Ca2+ involved in the regulation of cAMP-dependent and tyrosine phosphorylation pathways required for sperm capacitation.Fil: Navarrete, Felipe A.. University of Massachusetts; Estados UnidosFil: García Vázquez, Francisco A.. University of Massachusetts; Estados Unidos. Universidad de Murcia; EspañaFil: Alvau, Antonio. University of Massachusetts; Estados UnidosFil: Escoffier, Jessica. University of Massachusetts; Estados UnidosFil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; MéxicoFil: Salicioni, Ana M.. University of Massachusetts; Estados UnidosFil: Darszon, Alberto. Universidad Nacional Autónoma de México; MéxicoFil: Visconti, Pablo E.. University of Massachusetts; Estados Unido

The feeding of American plaice (Hippoglossoides platessoides), Redfish (Sebastes marinus) and Cod (Gadus morhua) in the Flemish Cap during July 1988

A randomly stratified bottom-trawl survey was realized in the Flemish Cap (NAFO Div. 3M) in July 1988. The stomach contents of three species were studied: 320 of American plaice (Hippoglossoides platessoides), 203 of redfish (Sebastes Marinus) and 468 of cod (Gadus morhua). The cod specimens were classified taking into account age groups; in the case of other species by length classes. The food items, the prey occurrence index and the diversity index were determined too. The Schoener overlap index was calculated to study the intraspecific diet overlap in cod and the interspecific diet overlap between the three species. The relation between predator size (cod) and prey size (redfish) was also studied

Iron deprivation enhances transcriptional responses to in vitro growth arrest of Mycobacterium tuberculosis

The establishment of Mycobacterium tuberculosis (Mtb) long-term infection in vivo depends on several factors, one of which is the availability of key nutrients such as iron (Fe). The relation between Fe deprivation inside and outside the granuloma, and the capacity of Mtb to accumulate lipids and persist in the absence of growth is not well understood. In this context, current knowledge of how Mtb modifies its lipid composition in response to growth arrest, depending on iron availability, is scarce. To shed light on these matters, in this work we compare genome-wide transcriptomic and lipidomic profiles of Mtb at exponential and stationary growth phases using cultures with glycerol as a carbon source, in the presence or absence of iron. As a result, we found that transcriptomic responses to growth arrest, considered as the transition from exponential to stationary phase, are iron dependent for as many as 714 genes (iron-growth interaction contrast, FDR <0.05), and that, in a majority of these genes, iron deprivation enhances the magnitude of the transcriptional responses to growth arrest in either direction. On the one hand, genes whose upregulation upon growth arrest is enhanced by iron deprivation were enriched in functional terms related to homeostasis of ion metals, and responses to several stressful cues considered cardinal features of the intracellular environment. On the other hand, genes showing negative responses to growth arrest that are stronger in iron-poor medium were enriched in energy production processes (TCA cycle, NADH dehydrogenation and cellular respiration), and key controllers of ribosomal activity shut-down, such as the T/A system mazE6/F6. Despite of these findings, a main component of the cell envelope, lipid phthiocerol dimycocerosate (PDIM), was not detected in the stationary phase regardless of iron availability, suggesting that lipid changes during Mtb adaptation to non-dividing phenotypes appear to be iron-independent. Taken together, our results indicate that environmental iron levels act as a key modulator of the intensity of the transcriptional adaptations that take place in the bacterium upon its transition between dividing and dormant-like phenotypes in vitro

Mercados municipales de Zapopan

Documento final del Proyecto de Aplicación Profesional en el que se presenta una investigación realizada en tres mercados del ayuntamiento de Zapopan: San Isidro, Francisco Sarabia y Tesistán. El objetivo de la investigación fue identificar las problemáticas, hacer propuestas y colaborar en la planeación de estrategias para mejorar las condiciones de los mercados y lograr un impacto positivo que se vea reflejado en las ventas. Para la investigación, se visitaron los mercados y se aplicaron encuestas a los locatarios, consumidores y administradores. La muestra que se usó para sacar el número de encuestas fue infinita con un nivel de confianza del 90 por ciento y un índice de error del 5 por ciento. En este proyecto trabajaron alumnos de las licenciaturas en Mercadotecnia, Administración de Empresas y Administración Financiera.ITESO, A.C

ALIMENTACIÓN A BASE DE FORRAJES EN FINCAS LECHERAS DEL MUNICIPIO FLORENCIA, CIEGO DE ÁVILA / FEEDING WITH THE USE OF FORAGES IN DAIRY FARMS IN THE MUNICIPALITY OF FLORENCIA, CIEGO DE ÁVILA

El presente trabajo tiene como objetivo caracterizar la alimentación con el uso forrajes en las fincas lecheras del municipio Florencia, a partir de la clasificación de estos sistemas. Se estudiaron 62 fincas con información cuantitativa según variables físicas, estructurales, productivas y de eficiencia. Se utilizaron los métodos de componentes principales y conglomerados jerárquicos. Se clasificaron las fincas según porcentaje de área de forrajes y se utilizó la variable cantidad de vacas para clasificar dentro de cada escala de utilización de forrajes. Se obtuvieron tres grupos de fincas: con menos de 3 %, entre 3 % y 10 % y más de 10 % del área para forrajes y dentro de cada uno de estos grupos se obtuvieron grupos de fincas con menos de 11, entre 11 y 25 y más de 25 vacas. Se encontraron diferencias en la utilización de forrajes como estrategia de alimentación en los grupos de fincas. A medida que se incrementó el porciento de áreas forrajeras promedio en las fincas, fue superior la producción de leche anual, por total de vacas y por hectárea. El 44,1 % de la variabilidad de la producción estuvo relacionada con los indicadores que definen el componente tecnológico, donde hay que tener en cuenta la extensión y el porcentaje que ocupan las áreas de forrajes. En los grupos de fincas que utilizaron 10 % o menos del área total para establecer forrajes, se encontró una relación negativa entre la carga y el porcentaje de vacas en ordeño

ACCIÓN INHIBITORIA DE PROBIÓTICOS SOBRE EL CRECIMIENTO AXÉNICO IN VITRO DEEntamoeba histolytica

Entamoeba histolytica es el protozoario parásito cosmopolita causante de la amibiasis y sus múltiples manifestaciones clínicas afectando con mayor frecuencia a los países en vías de desarrollo en donde existe hacinamiento y condiciones sanitarias deficientes e inadecuadas; en México representa un problema de saludpública por su frecuencia, morbilidad, mortalidad y fácil dispersión; para el control de la amibiasis y sus manifestaciones clínicas, se administra metronidazol como droga de elección, sin embargo este fármaco produce efectos secundarios indeseables. Reportes recientes indican que cultivos in vitro de E. histolytica han desarrollado resistencia a este fármaco. En este trabajo evaluamos el efecto de 1, 10, 20 y 100 mg/mL de liofilizados de medios condicionados con probióticos (Lactobacillus acidophilus, Lactobacillus casei y Lactococcus lactis) sobre el crecimiento axénico in vitro de E. histolytica. Las concentraciones ensayadas mostraron diferencia estadística significativa (ANOVA p=0.05) como inhibidores del crecimiento in vitro de Entamoeba histolytica HM1-IMSS. Estos resultados abren la posibilidad de contar con una alternativa nutricional para la prevención y tratamiento de la amibiasis sin presentar efectos secundarios indeseables.Palabras claves: Entamoeba histolytica, Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, cultivo axénico, probióticos.Entamoeba histolytica, Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, axenic culture, probiotics